The interview, which aired on Oprah's Next Chapter on Oprah's OWN network at the weekend, marked the first time the 30-year-old had commented on her family's killer, William Balfour, since he was handed three life sentences in jail in late July.
"For the most part, it's not his fault. It's what he was taught, how he was brought up," Jennifer said of her former brother-in-law.
Jennifer, mother of three-year-old David Daniel Otunga Jr, then addressed William directly.
"You never had a chance. Had you had the love my mother gave us, or the background, you know, that some have, then you would've stood a chance."
Watch an excerpt of the interview above to hear what Jennifer said about her grief, and why she hasn't visited the grave site since her family were buried.
Jennifer's sister Julia, the ex-wife of the killer, also spoke to Oprah about blaming herself for the shocking shooting of her family at their home in Chicago.
"Once I did begin to see who he really was, I tried to get out. That was his promise: 'If you leave me, I'll kill you. I'll kill your family first'," Julia said.
"I left. And he did what he said he would."
As well as talking about the family tragedy, Jennifer opened up about getting lip injections to fix a "dent" in her lip while she filmed Dreamgirls.
"When I did Dreamgirls, I actually had to get my dent right here injected because it was affecting the lighting," she revealed.
"And when I got it done, the doctor was like 'I do not want to do this. Do you realise people come in here and pay to have the lips you have naturally'. And I was like, 'I don't want to but I have to for the movie!'"
But things are looking up for Jennifer. She is currently planning her wedding to fiancé David Otunga, and has a number of projects on the go, including a guest role on the hit show Smash. In 2013, she will receive a star on the Hollywood Walk of Fame.
Check out Jennifer's original American Idol audition tape from 2004 in our slideshow of superstars' reality TV auditions: